Abstract:
Background: L. sativum, are traditionally used for the treatment of various diseases and
thought to have medicinal value. Isolates from many part of the world is now multidrug
resistant. Therefore, there is an urgent need to look for and test an alternative herbal drug.
Objective: The present study aimed to evaluate the antibacterial activity of L. Sativum seed
extract against multi drug resistant (MDR) and sensitive Pseudomonas aeruginosa clinical
isolates.
Subjects and Methods: An ethanolic and aqueous stock extracts were prepared from L.
sativum seed plant then serial dilutions were prepared and the obtained concentrations (50, 25,
12.5 and 6.2 mg/ml) were tested against 30 multidrug-resistant and 35 sensitive clinical
isolates of Pseudomonas aeruginosa using wells diffusion method.
Results: It was found that L. sativum seed extracts had antimicrobial activity against MDR
and sensitive isolates at different concentrations of 100, 50 and 25 according to the mean ±
SD (standard deviation) of the maximum zones of inhibition. The total number of isolates that
were sensitive to both extracts were 49/130 (37%) which represented 17/60 (28.3%) MDR
and 32/70 (45.7%) sensitive isolates. The aqueous extract exhibited more inhibitory effect
than ethanolic extract 43 (66%) vs. 6 (9%) against the examined isolates (n=65).
Conclusion: The study concluded that the L. sativum extracts had an antibacterial activity
against the susceptible and MDR isolates which may enable it to be used an alternative
treatment for medicinal purposes.
